WT1

  • Official Full Name

    Wilms tumor 1

  • Overview

    Wilm’s Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma.
  • Synonyms

    WT1; Wilms tumor 1; GUD; Wilms tumor protein; AWT1; WAGR; WIT 2; amino-terminal domain of EWS|last three zinc fingers of the DNA-binding domain of WT1; WT33; NPHS4; WIT-2; EWS-WT1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Mouse
  • Rat
  • E. coli
  • E.coli
  • HEK293
  • Insect Cell
  • Mammalian Cell
  • Sf9 Insect Cell
  • Yeast
  • Flag
  • His
  • Fc
  • Avi
  • GST
  • Myc
  • SUMO
  • Non
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human WT1-1210H Active Recombinant Human Wilms Tumor 1 Sf9 Insect Cell Non
Human WT1-1199H Active Recombinant Human WT1, Flag-tagged Insect Cell Flag 1-446
Human WT1-1402H Recombinant Human WT1 protein, His & GST-tagged E.coli His&GST Leu310~Leu449
Human WT1-30863TH Recombinant Human WT1, FLAG-tagged Flag
Human WT1-17H Recombinant Human WT1 protein, His-tagged E.coli His Leu310~Leu449
Human WT1-625H Recombinant Human WT1 Protein, His-tagged E.coli His
Human WT1-626H Recombinant Human WT1 Protein, His-tagged E.coli His
Human WT1-278HCL Recombinant Human WT1 293 Cell Lysate HEK293 Non
Human WT1-3769H Recombinant Human WT1 protein, His-SUMO-tagged E.coli His&SUMO 1-449aa
Human WT1-5272H Recombinant Human WT1 protein, His-tagged E.coli His 1-299aa
Human WT1-1861H Recombinant Human WT1 Protein (1-449 aa), His-tagged Yeast His 1-449 aa
Human WT1-07HFL Recombinant Full Length Human WT1 Protein, N-His-tagged E.coli His
Human WT1-589H Recombinant Human WT1 Protein, His-tagged E.coli His Met1-Ser150
Human WT1-6572H Recombinant Human WT1 Protein (Thr317-Gln446), N-His tagged E.coli His Thr317-Gln446
Human WT1-001H Recombinant Human WT1 Protein, His-tagged E. coli His 127-249 aa
Mouse Wt1-15M Recombinant Mouse Wt1 protein E.coli Non Thr402-Ser415
Mouse WT1-18590M Recombinant Mouse WT1 Protein Mammalian Cell His
Mouse Wt1-1162M Recombinant Mouse Wt1 protein, His&Myc-tagged E.coli His&Myc 1-449aa
Mouse WT1-10211M Recombinant Mouse WT1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Mouse WT1-10211M-B Recombinant Mouse WT1 Protein Pre-coupled Magnetic Beads HEK293
Rat WT1-6605R Recombinant Rat WT1 Protein Mammalian Cell His
Rat WT1-6261R Recombinant Rat WT1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Rat WT1-6261R-B Recombinant Rat WT1 Protein Pre-coupled Magnetic Beads HEK293
Chicken WT1-6769C Recombinant Chicken WT1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
WT1-7.jpg

Fig1. WT1 is an important factor for AML maintenance in multiple subtypes. (Sandeep Potluri, 2021)

What is WT1 protein?

WT1 (WT1 transcription factor) gene is a protein coding gene which situated on the short arm of chromosome 11 at locus 11p13. The WT1 gene is normally expressed in fetal kidney and mesothelioma. WT1, the product of WT1 gene, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. The WT1 protein is consisted of 449 amino acids and its molecular mass is approximately 49.2 kDa.

What is the function of WT1 protein?

WT1 is a transcription factor that plays an important role in cellular development and cell survival. With the inclusion of exon 5, WT-1 (+KTS) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also colocalizes with splicing factor in a speckled nuclear particle, suggesting that WT-1 protein may function as both a transcription factor and a splicing regulator. At the same time, it has a tumor suppressor as well as an oncogenic role in tumor formation.

WT1 Related Signaling Pathway

The protein encoded by WT1 gene is a bidirectional transcription factor, which has dual functions of inhibiting tumor growth and activating oncogene transcription. WT1 may negatively regulate the Wnt/β-catenin signaling pathway, and is also regulated by many signaling pathways, such as PI3K/Akt and MAPK/ERK signaling pathways. It is also involved in transcriptional regulation of some genes as a transcription factor.

WT1 Related Diseases

Wilm's tumor (WT1) is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. Mutations in the WT1 gene are also associated with frasier syndrome (FS). FS is an X-linked genetic disorder in which mutations in the WT1 gene cause tubular dysplasia and gonadal hypoplasia. Other related diseases include: nephroblastoma, skeletal system diseases, reproductive system diseases, etc. In addition, it is highly expressed in all types of solid tumors and leukemias, especially acute myeloid leukemia (AML).

Bioapplications of WT1

WT1 can be used as a tumor marker to diagnose and monitor the effect of tumor therapy. In addition, anti-sense oligonucleotides, siRNA and other drugs targeting WT1 are also being studied and are expected to become a new tumor therapy.

Case Study 1: Chunhong Wang, 2022

WT1-3.jpg

Fig1. Overexpression of miR-671-5p impairs but knockdown of miR-671-5p protects podocyte integrity in vitro.

Case Study 2: Zhuna Wu, 2023

WT1-4.jpg

Fig2. Significantly high WT1 expression was observed in cervical caner tissues with relapse.

WT1 involved in several pathways and played different roles in them. We selected most pathways WT1 participated on our site, such as Transcriptional misregulation in cancer, which may be useful for your reference. Also, other proteins which involved in the same pathway with WT1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Transcriptional misregulation in cancerTLX3;CCNA1;MEF2C;RXRB;TCF3;HIST1H3E;PROM1;FUT8;IGF1R

WT1 has several biochemical functions, for example, C2H2 zinc finger domain binding, RNA binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by WT1 itself. We selected most functions WT1 had, and list some proteins which have the same functions with WT1. You can find most of the proteins on our site.

Function Related Protein
C2H2 zinc finger domain bindingEHMT1;TAF9;ZXDC;EBF1;LEF1;GATA2;GATA1;THAP7;WT1
RNA bindingMCTS2;ZNF385C;EXOSC7;KIAA0430;DOM3Z;CNOT7;PKZ;ENDOU;KHDRBS1
protein bindingMPZL1;SMCR7;GRIN2D;BLOC1S1;RCHY1;PDIK1L;MTSS1;ACAP1;SLC25A23
sequence-specific DNA bindingSIX9;RORCA;NKX1.2LA;NKX2.2B;FOXA;NR1H3;HOXB10A;NFIL3-2;CREMB
transcription factor activity, sequence-specific DNA bindingZNF311;ZNF215;GRHL2;FOXA2;HCFC1;ZSCAN23;JUNBA;DBPB;HIVEP2A
transcription regulatory region DNA bindingBRD7;HOXA13;NKX2;RELA;DDIT3;SOX5;CTCF;GRHL1;FOXM1
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific bindingNFAT5;SOX12;POU3F2;CREB3L2;SREBF1;ZFAT;NR4A3;TXK;ZNF750
zinc ion bindingGATA1;ZFP276;MORC3B;CPB1;RNF170;ZDHHC3;ZDHHC16;RORCB;PIAS4

WT1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with WT1 here. Most of them are supplied by our site. Hope this information will be useful for your research of WT1.

KRTAP10-8; KRT40; DVL3; NPM3; ZNF205; DIRAS3; NAT2; CCL5; KLK7; MDM2; TAOK1; CASZ1; THRSP

Research Area

Related articles

Qi, XW; Zhang, F; et al. Wilms' tumor 1 (WT1) expression and prognosis in solid cancer patients: a systematic review and meta-analysis. SCIENTIFIC REPORTS 5:-(2015).
Sasine, JP; Schiller, GJ; et al. Emerging strategies for high-risk and relapsed/refractory acute myeloid leukemia: Novel agents and approaches currently in clinical trials. BLOOD REVIEWS 29:1-9(2015).
  • Reviews
  • Q&As

Customer Reviews (3)

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Reviews
10/10/2020

    It has been widely employed in protein electron microscopy structure analysis, where it has demonstrated remarkable efficacy.

    10/25/2019

      Broad applicability make it a valuable asset for a wide range of research endeavors.

      05/07/2017

        Researchers can rely on the WT1 Protein to deliver reliable and reproducible results, facilitating robust data interpretation and scientific advancements.

        Q&As (5)

        Ask a question
        What therapeutic strategies are being explored for cancers with abnormal WT1 expression? 05/25/2021

        Targeted therapies and immunotherapies aimed at inhibiting or modulating WT1 expression are being investigated for their potential in cancer treatment.

        In what other cancers is the WT1 protein targeted for diagnostic purposes? 09/04/2019

        Apart from Wilms' tumor, WT1 is examined in leukemia, mesothelioma, and some solid tumors as a diagnostic marker.

        Can WT1 be a target for personalized cancer therapy? 04/01/2019

        Yes, understanding the WT1 status in a patient's cancer can guide the development of personalized treatment plans, potentially improving outcomes.

        Are there any ongoing clinical trials focused on the WT1 protein and cancer? 02/09/2019

        Yes, there are several clinical trials exploring the role of WT1 as a therapeutic target and its potential in cancer treatment.

        How does the WT1 protein contribute to the prognosis of cancer patients? 10/23/2017

        High levels of WT1 expression are often associated with poor prognosis in various cancers, indicating a more aggressive disease course.

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