"Wt1" Related Products


Recombinant Mouse Wt1 protein

Cat.No.: Wt1-15M
Product Overview: Recombinant Mouse Wt1(Thr402-Ser415) was expressed in E. coli.
Description: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene, however, the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites, resulting in different isoforms.
Source: E. coli
Species: Mouse
Form: PBS, pH7.4.
Protein length: Thr402-Ser415
Purity: >90%
Applications: Immunogen; SDS-PAGE.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name: Wt1 Wilms tumor 1 homolog [ Mus musculus ]
Official Symbol: Wt1
Synonyms: WT1; Wilms tumor 1 homolog; Wilms tumor protein homolog; Wilms tumor homolog; Wt-1; D630046I19Rik;
Gene ID: 22431
mRNA Refseq: NM_144783
Protein Refseq: NP_659032
Chromosome Location: 2 E2-E3; 2 55.06 cM
Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function: C2H2 zinc finger domain binding; C2H2 zinc finger domain binding; DNA binding; RNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; double-stranded DNA binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding; zinc ion binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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