| Species : |
Human |
| Source : |
Sf9 Cells |
| Tag : |
Non |
| Description : |
WT-1, the product of Wilms' Tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT-1 protein. Such a complex posttranscriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms' tumor. With the inclusion of exon 5, WT-1 (+KTS) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also colocalizes with splicing factor in a speckled nuclear particle, suggesting that WT-1 protein may function as both a transcription factor and a splicing regulator. The WT-1 protein (residue 1-449, including exon 5 and KTS) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. |
| Form : |
Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
| Purity : |
≥95% by SDS-PAGE. |
| Activity : |
1 ng is the amount sufficient for a gel mobility shift assay in a 20 μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay. |
| Usage : |
For in vitro use only. |
| Storage : |
Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles. |
| Publications : |
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