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| Cat.No.: | Kit-1886 |
| Product Overview: | TNKS2 catalyzes the NAD-dependent addition of poly (ADP-ribose) to the substrate proteins. |
| Size: | 96 reactions |
| Description: | The TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 2 (TNKS2) activity for screening and profiling applications. The key to the TNKS2 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+ substrate is incubated with an assay buffer that contains the TNKS2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. |
| Applications: | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
| Storage: | 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
| Kit Components: | TNKS2: 2 µg; -80°C 5 x histone mixture: 1 ml; -80°C10x Assay Mixture Containing Biotinylated Substrate: 300 μl; -80°C10x PARP Assay Buffer: 1 ml; -80°CBlocking buffer: 25 ml; +4°CStreptavidin-HRP: 100 μ; +4°CHRP chemiluminescent substrate (2 components): 6 ml each; +4°C 96-well module: 1; Room Temperature |
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