Active Recombinant Cynomolgus Monkey IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253) Protein
|Product Overview :||Recombinant Cynomolgus Monkey IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253) protein without tag was expressed in Human embryonic kidney cell.|
- Gene Information
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|Description :||This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.|
|Form :||Disulfide-linked heterodimer|
|Bio-activity :||Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 0.15-0.9 ng/mL.|
|Molecular Mass :||Predicted Molecular Mass: 58 kDa
SDS-PAGE: 65-78 kDa, under reducing conditions
|Protein Length :||IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253)|
|N-terminal Sequence Analysis :||Ile23|
|Endotoxin :||<0.10 EU/μg of the protein by the LAL method.|
|Purity :||>95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer :||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution :||Reconstitute at 100 μg/mL in PBS.|
|Shipping :||The product is shipped at ambient temperature.|
|Gene Name :||IL12A interleukin 12A [ Homo sapiens (human) ]|
|Official Symbol :||IL12A|
|Synonyms :||IL12A; interleukin 12A; P35; CLMF; NFSK; NKSF1; IL-12A|
|Gene ID :||3592|
|mRNA Refseq :||NM_000882|
|Protein Refseq :||NP_000873|
|UniProt ID :||P29459|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Fluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)Write a review
Effective in Th1 cell differentiation. -
High specificity in ELISA. -
Stable under storage. -
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