Active Recombinant Cynomolgus Monkey IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253) Protein

Cat.No. : IL12-22H
Product Overview : Recombinant Cynomolgus Monkey IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253) protein without tag was expressed in Human embryonic kidney cell.
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  • Gene Information
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Description : This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Source : HEK293
Species : Human
Form : Disulfide-linked heterodimer
Bio-activity : Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 0.15-0.9 ng/mL.
Molecular Mass : Predicted Molecular Mass: 58 kDa
SDS-PAGE: 65-78 kDa, under reducing conditions
Protein Length : IL-12p40 (Ile23-Ser328) and IL-12p35 (Arg57-Ser253)
N-terminal Sequence Analysis : Ile23
Endotoxin : <0.10 EU/μg of the protein by the LAL method.
Purity : >95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution : Reconstitute at 100 μg/mL in PBS.
Shipping : The product is shipped at ambient temperature.
Gene Name : IL12A interleukin 12A [ Homo sapiens (human) ]
Official Symbol : IL12A
Synonyms : IL12A; interleukin 12A; P35; CLMF; NFSK; NKSF1; IL-12A
Gene ID : 3592
mRNA Refseq : NM_000882
Protein Refseq : NP_000873
MIM : 161560
UniProt ID : P29459

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Q&As (10)

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How does fluorescence microscopy track IL12 internalization and intracellular trafficking? 11/21/2022

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines? 11/13/2022

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles? 01/30/2022

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements? 01/27/2022

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide? 09/02/2021

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments? 03/31/2021

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context? 02/22/2021

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry? 12/18/2020

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks? 10/10/2020

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation? 03/19/2020

Transgenic models elucidate IL12's roles in immune response regulation.

Customer Reviews (3)

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    Effective in Th1 cell differentiation. -


      High specificity in ELISA. -


        Stable under storage. -

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