How does fluorescence microscopy track IL12 internalization and intracellular trafficking?
Fluorescence microscopy tracks IL12 internalization and trafficking.
What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
What strategies assess IL12's polarization of T cell subsets and cytokine profiles?
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
How are reporter gene assays tailored to study IL12's promoter activity and response elements?
Reporter gene assays analyze IL12's promoter activity and response elements.
What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?
Super-resolution imaging details IL12's cellular distribution and dynamics.
What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?
3D cell culture models reveal IL12's effects on tissue microenvironments.
How do protein-protein interaction assays decipher IL12's binding partners in cellular context?
Co-localization studies and co-IP assays identify IL12's binding partners.
What methods elucidate IL12's receptor complex formation and stoichiometry?
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Could you explain how transgenic models help dissect IL12's roles in immune response modulation?
Transgenic models elucidate IL12's roles in immune response regulation.