Active Recombinant Rat Il12 Protein, His-tagged

• Cat.No.: Il12-258I
• Product Overview: Recombinant Rat Il12 Protein with a His tag was expressed in CHO.

Specification

• Description: Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
• Source: CHO
• Species: Rat
• Tag: His
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50< 0.1 ng/mL, measured in a cell proliferation assay using 2D6 cells.
• Molecular Mass: 26-28kDa (p35) and 42-45 kDa (p40), observed by reducing SDS-PAGE.
• AA Sequence: p35:RVIPVSGPAKCLNQSQNLLKTTDDM VRTAREKLKHYSCTAGDIDHEDITR DKTSTLEACLPLELHKNESCLATKE TSSIIRGSCLPPQKTSLMMTLCLGS IYEDLKMYQSEFQAINAALQSHNHQ QITLDRNMLMAIDELMRSLNHSGET LHQKAPMGEADPYRVKMKLCILLHA FSTRVMTINRVMNYLSSSHHHHHH
  p40:MWELEKDVYVVEVDWRPDAPGETVT LTCDSPEEDDITWTSDQRRGVIGSG KTLTITVREFLDAGQYTCHRGGETL SHSHLLLHKKENGIWSTEILKNFKN KTFLKCEAPNYSGRFTCSWLVHRNT DLKFNIKSSSSSPESRAVTCGRASL SAEKVTLNQRDYEKYSVACQEDVTC PTAEETLPIELVVEAQQQNKYENYS TSFFIRDIIKPDPPKNLQVKPLKNS QVEVSWEYPDSWSTPHSYFSLKFFV RIQRKKEKTKETEEECNQKGAFLVE KTSAEVQCKGANICVQAQDRYYNSS CSKWTCVPCRGRS
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant rat Interleukin-12 (IL-12), His remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rat Interleukin-12 (IL-12), His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Il12 Interleukin 12 [ Rattus norvegicus ]
• Official Symbol: Il12
• Synonyms: IL12; Interleukin 12
• Gene ID: 64546
• mRNA Refseq: NM_022611
• Protein Refseq: NP_072133
• UniProt ID: E9PU71

Reviews

01/07/2022

Effective in Th1 cell differentiation.

04/09/2020

High specificity in ELISA.

08/02/2018

Stable under storage.

Q&As

How does fluorescence microscopy track IL12 internalization and intracellular trafficking?

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles?

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements?

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context?

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry?

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation?

Transgenic models elucidate IL12's roles in immune response regulation.