Active Recombinant Mouse IL12, HIgG1 Fc-tagged
| Cat.No. : | IL12-002M |
| Product Overview : | The mouse IL-12 complex composed of the p40 subunit (NP_032377.1) (aa1-335) and the p35 subunit (NP_032377.1)(aa 23-215) is fused through a polypeptide linker to the Fc portion of mouse IgG2a was expressed in CHO cell. |
- Specification
- Gene Information
- Related Products
| Source : | CHO cell |
| Species : | Mouse |
| Tag : | HIgG1 Fc-tagged |
| Form : | Lyophilized from 0.2μm-filtered solution in PBS. |
| Bio-activity : | Measured in a cell proliferation assay using PHA-activated mouse splenocytes. |
| Molecular Mass : | 87 KDa |
| AA Sequence : | The mouse IL-12 complex composed of the p40 subunit (NP_032377.1) (aa1-335) and the p35 subunit (NP_032377.1)(aa 23-215) is fused through a polypeptide linker to the Fc portion of mouse IgG2a. |
| Endotoxin : | <0.06 eu/μg as determined by lal> |
| Purity : | ≥98%, by SDS-PAGE under reducing conditions. |
| Stability : | Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Reconstitution : | Reconstitute at 100μg/ml in sterile PBS. |
| Warning : | Avoid freeze/thaw cycles. |
| Gene Name : | IL-12 |
| Official Symbol : | IL12 |
| Synonyms : | IL12; IL12B; IL-12B |
| Gene ID : | 100860846 |
| mRNA Refseq : | NM_001285700 |
| Protein Refseq : | NP_001272629 |
| UniProt ID : | P68221 |
| Pathway : | African trypanosomiasis, organism-specific biosystem;Allograft rejection, organism-specific biosystem;Chagas disease (American trypanosomiasis), organism-specific biosystem |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionFluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)
Write a reviewEffective in Th1 cell differentiation. -
High specificity in ELISA. -
Stable under storage. -
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