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Active Recombinant Mouse IL12 protein, His-tagged

Cat.No. : IL12-28M
Product Overview : This product contains IL12A & IL12B heterodimer. A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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  • Gene Information
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Description : Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4.
Bio-activity : 1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA.
2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 2-8 ng/mL.
Molecular Mass : The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name : interleukin 12a [ Mus musculus (house mouse) ]
Official Symbol : Il12a
Synonyms : CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1;
NKSF2; P35; Il-12a; Il-12b; IL-12p35; Il-12p40; Il12p40; Ll12a; p35; p40
Gene ID : 16159
mRNA Refseq : NM_001159424.2
Protein Refseq : NP_001152896.1
UniProt ID : P43431

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does fluorescence microscopy track IL12 internalization and intracellular trafficking? 11/21/2022

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines? 11/13/2022

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles? 01/30/2022

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements? 01/27/2022

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide? 09/02/2021

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments? 03/31/2021

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context? 02/22/2021

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry? 12/18/2020

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks? 10/10/2020

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation? 03/19/2020

Transgenic models elucidate IL12's roles in immune response regulation.

Customer Reviews (3)

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Reviews
01/07/2022

    Effective in Th1 cell differentiation.

    04/09/2020

      High specificity in ELISA.

      08/02/2018

        Stable under storage.

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