Active Recombinant Cynomolgus/Rhesus macaque CD274 protein, Fc-tagged

• Cat.No.: CD274-1947C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CD274 protein(Phe 19 - Arg 238), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Loaded Cynomolgus / Rhesus macaque PD-L1, Fc Tag on Protein A Biosensor, can bind Cynomolgus PD-1, His Tag with an affinity constant of 4.2 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Cynomolgus PD-1, His Tag on HIS1K Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag with an affinity constant of 28.3 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.8 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Phe 19 - Arg 238
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.