Active Recombinant Mouse CD274 Protein, Fctagged

• Cat.No.: CD274-77M
• Product Overview: PD-L1 protein dimer contains a PD-L1 extracellular domain (UniProt# Q9EP73) fused with a proprietary cis-dimer motif followed by a Fc tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293T
• Tag: Fc
• Protein Length: 19-239 aa
• Description: Programmed death-ligand 1 (PD-L1), is a Type I transmembrane protein in the immunoglobulin superfamily and a member of the B7 Family of ligands. PD-L1 is also known as cluster of differentiation 274 (CD274), B7 homolog 1 (B7H1, B7-H1), PDCD1L1, PDCD1LG1, and CD274 molecule. PD-L1 contains an extracellular domain with a distal immunoglobulin V-like (Ig-V-like) domain and proximal immunoglobulin C-like (Ig-C-like) domain, a transmembrane domain, and a cytoplasmic domain. PD-L1 is expressed on T cells, NK cells, macrophages, myeloid DCs, B cells, epithelial cells, and vascular endothelial cells. PD-L1 serves as an immunosuppressive ligand for PD-1 and the overexpression of PD-L1 on many tumor cells can prevent the immune system from attacking tumors. Inhibition of the interaction between PD-1 and PD-L1 can enhance antitumor activity, which has led to a new class of drugs called PD-1 inhibitors to activate the immune system and treat certain types of cancer. PD-L1 is highly expressed in a variety of malignancies, particularly lung cancer. PD-L1 exists as both a monomer and a dimer. Therefore, a recombinant protein mimicking the PD-L1 dimer conformation can be crucial for cancer therapeutic discovery. Mouse PD-L1, the murine homolog of human Programmed Death-Ligand 1 (PD-L1), is a critical immune checkpoint protein involved in regulating immune responses and is indispensable for studying immune checkpoint biology and immunotherapy. It is a species-specific tool essential for basic research, translational research and preclinical studies.
• Molecular Mass: 102 kDa
• Homodimer/Heterodimer: Homodimer
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA and SPR for PD-L1-specific antibody binding assays. Suggested Applications: SPR for PD-L1 and PD-1 protein binding assays. BLI for PD-L1-specific antibody and PD-1 protein binding assays. Animal immunization, RUO.
• Storage: At -80 centigrade
• Storage Buffer: 0.2μm filtered PBS, pH 7.4, no preservatives
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name 2: Cd274 CD274 antigen [ Mus musculus (house mouse) ]
• Official Symbol 2: Cd274
• Synonyms 2: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID 2: 60533
• mRNA Refseq 2: NM_021893
• Protein Refseq 2: NP_068693
• UniProt ID 2: Q9EP73