Active Recombinant Human CCL1 Protein (74 aa)

• Cat.No.: CCL1-111C
• Product Overview: Recombinant Human CCL1 Protein without tag was expressed in E. coli.

Specification

• Description: Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a γ/δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract total human T cell population using a concentration range of 10.0-100.0 ng/mL, corresponding to a Specific Activity of ＞1 × 10^4 IU/mg.
• Molecular Mass: 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
• Protein length: 74
• AA Sequence: SKSMQVPFSRCCFSFAEQEIPLRAI LCYRNTSSICSNEGLIFKLKRGKEA CALDTVGWVQRHRKMLRHCPSKRK
• Endotoxin: Less than 1 EU/mg of rHuI-309/CCL1 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CCL1 chemokine (C-C motif) ligand 1 [ Homo sapiens ]
• Official Symbol: CCL1
• Synonyms: CCL1; chemokine (C-C motif) ligand 1; SCYA1, small inducible cytokine A1 (I 309, homologous to mouse Tca 3); C-C motif chemokine 1; I 309; inflammatory cytokine I 309; P500; SISe; T lymphocyte secreted protein I 309; TCA3; inflammatory cytokine I-309; small-inducible cytokine A1; T lymphocyte-secreted protein I-309; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); I-309; SCYA1
• Gene ID: 6346
• mRNA Refseq: NM_002981
• Protein Refseq: NP_002972
• MIM: 182281
• UniProt ID: P22362

Reviews

11/14/2022

Its exceptional purity and integrity ensure clear and reliable bands, enabling accurate detection and quantification of target proteins.

12/03/2019

Its stability and structural integrity make it an ideal candidate for studying protein structures at high resolution.

11/11/2019

I am delighted to highly recommend the aroE Protein for researchers seeking excellent performance in Western blotting (WB) experiments and protein electron microscopy structure analysis

Q&As

How does CCL1 contribute to the pathogenesis of inflammatory conditions?

CCL1 promotes the recruitment of immune cells to inflamed tissues, exacerbating inflammation and contributing to the pathogenesis of various inflammatory conditions.

Are there any ongoing clinical trials targeting CCL1 for therapeutic intervention?

Several clinical trials are exploring the therapeutic potential of targeting CCL1 in autoimmune diseases and inflammatory disorders to modulate immune responses.

How does CCL1 interact with other immune modulators, and what implications does this have for drug development?

CCL1 interacts with various immune modulators, and understanding these interactions is crucial for developing targeted therapies that can modulate the immune response more effectively.

In what ways does CCL1 participate in the regulation of immune responses?

CCL1 regulates immune responses by influencing the migration and activation of specific immune cells, modulating the overall immune system's activity.

How does CCL1 influence the migration of specific immune cell types, and how is this mechanism exploited in clinical applications?

CCL1 selectively influences the migration of specific immune cell types, and understanding this mechanism is essential for developing therapies that target specific immune responses in various diseases.