Active Recombinant human CD4 protein (26-390aa), C-hIgG/His-tagged

• Cat.No.: CD4-39H
• Product Overview: Recombinant human CD4 protein (26-390aa), fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
• Source: HEK293
• Species: Human
• Tag: His&Fc
• Form: Liquid
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When cells are added to human CD4 coated plates. The ED50 range ≤ 10 μg/mL.
• Molecular Mass: 67.7kDa (604aa)
• Protein length: 26-390
• AA Sequence: KKVVLGKKGDTVELTCTASQKKSIQ FHWKNSNQIKILGNQGSFLTKGPSK LNDRADSRRSLWDQGNFPLIIKNLK IEDSDTYICEVEDQKEEVQLLVFGL TANSDTHLLQGQSLTLTLESPPGSS PSVQCRSPRGKNIQGGKTLSVSQLE LQDSGTWTCTVLQNQKKVEFKIDIV VLAFQKASSIVYKKEGEQVEFSFPL AFTVEKLTGSGELWWQAERASSSKS WITFDLKNKEVSVKRVTQDPKLQMG KKLPLHLTLPQALPQYAGSGNLTLA LEAKTGKLHQEVNLVVMRATQLQKN LTCEVWGPTSPKLMLSLKLENKEAK VSKREKAVWVLNPEAGMWQCLLSDS GQVLLESNIKVLPTW
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 o +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens (human) ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4 antigen (p55) , T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730

Reviews

01/08/2031

The CD4 proteins purchased from your company is suitable for ELISA.

02/06/2023

Protein products are put into experiments with good effect and reliable experimental results.

01/22/2022

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

Q&As

What is the connection between CD4 and glycoprotein GP120?

CD4 binds to glycoprotein GP120.

Where is CD4 primarily expressed?

CD4 is mainly expressed in T cells and thymocytes.

Where is CD4 localized?

CD4 is localized in the cell membrane.