Recombinant Mouse CD4 cell lysate

• Cat.No.: CD4-2580MCL
• Product Overview: Mouse CD4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Cd4 CD4 antigen [ Mus musculus ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); L3T4; Ly-4
• Gene ID: 12504
• mRNA Refseq: NM_013488
• Protein Refseq: NP_038516
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem
• Function: enzyme binding; glycoprotein binding; protein homodimerization activity; protein kinase binding; zinc ion binding

Reviews

01/08/2031

The CD4 proteins purchased from your company is suitable for ELISA.

02/06/2023

Protein products are put into experiments with good effect and reliable experimental results.

01/22/2022

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

Q&As

What is the connection between CD4 and glycoprotein GP120?

CD4 binds to glycoprotein GP120.

Where is CD4 primarily expressed?

CD4 is mainly expressed in T cells and thymocytes.

Where is CD4 localized?

CD4 is localized in the cell membrane.