Recombinant Ferret CD4 cell lysate

Cat.No. : CD4-1865FCL
Product Overview : Ferret CD4 / Leu-3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Ferret
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD4 CD4 molecule [ Mustela putorius furo ]
Official Symbol : CD4
Synonyms : CD4 molecule
Gene ID : 101684487
mRNA Refseq : XM_004766661
Protein Refseq : XP_004766718
Chromosome Location : chromosome: Un

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (3)

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What is the connection between CD4 and glycoprotein GP120? 11/09/2022

CD4 binds to glycoprotein GP120.

Where is CD4 primarily expressed? 02/26/2022

CD4 is mainly expressed in T cells and thymocytes.

Where is CD4 localized? 03/14/2021

CD4 is localized in the cell membrane.

Customer Reviews (3)

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Reviews
01/08/2031

    The CD4 proteins purchased from your company is suitable for ELISA.

    02/06/2023

      Protein products are put into experiments with good effect and reliable experimental results.

      01/22/2022

        The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

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