Active Recombinant Human FGF19 Protein (195 aa)

• Cat.No.: FGF19-121F
• Product Overview: Recombinant Human FGF19 Protein without tag was expressed in E. coli.

Specification

• Description: Fibroblast growth factor 19 (FGF19) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Unlike most FGFs which bind to and activate more than one FGF receptor, FGF19 is a specific ligand for FGF R4.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150 ng/mL, corresponding to a Specific Activity of ＞6.7× 10^3 IU/mg.
• Molecular Mass: Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.
• Protein Length: 195
• AA Sequence: MRPLAFSDAGPHVHYGWGDPIRLRH LYTSGPHGLSSCFLRIRADGVVDCA RGQSAHSLLEIKAVALRTVAIKGVH SVRYLCMGADGKMQGLLQYSEEDCA FEEEIRPDGYNVYRSEKHRLPVSLS SAKQRQLYKNRGFLPLSHFLPMLPM VPEEPEDLRGHLESDMFSSPLETDS MDPFGLVTGLEAVRSPSFEK
• Endotoxin: Less than 1 EU/mg of rHuFGF-19 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: FGF19 fibroblast growth factor 19 [ Homo sapiens ]
• Official Symbol: FGF19
• Synonyms: FGF19; fibroblast growth factor 19; FGF-19
• Gene ID: 9965
• mRNA Refseq: NM_005117
• Protein Refseq: NP_005108
• MIM: 603891
• UniProt ID: O95750

Reviews

01/14/2022

Top-notch protein quality! It's our go-to for reliable and reproducible results.

09/08/2020

This product simplifies our research. It's a reliable tool that we can count on.

05/11/2018

If you're in the lab, you need this product. It streamlines processes and is a lab necessity.

Q&As

How is FGF19 gene expression regulated, and what factors influence its secretion and activity in various tissues?

FGF19 expression is regulated by bile acids and other factors. Its secretion and activity are influenced by nutrient availability, signaling pathways, and interactions with bile acid receptors.

Can you discuss the potential therapeutic applications of targeting FGF19 in metabolic disorders, and what challenges exist in developing such interventions?

Targeting FGF19 holds therapeutic potential for metabolic disorders. Challenges include achieving tissue-specific effects and understanding the long-term consequences of manipulating FGF19 signaling.

Can you explain the role of FGF19 in the enterohepatic circulation of bile acids and its implications for metabolic health?

FGF19 regulates bile acid synthesis in the liver and influences the enterohepatic circulation, impacting metabolic health by modulating lipid and glucose metabolism.

What is the role of FGF19 in the regulation of gut hormone secretion, and how does this contribute to its effects on metabolic processes?

FGF19 influences gut hormone secretion, including the release of glucagon-like peptide-1 (GLP-1). This regulation contributes to its metabolic effects, such as enhanced insulin sensitivity and reduced food intake.

What is the significance of FGF19 in metabolic diseases, and how does dysregulation contribute to conditions like obesity and non-alcoholic fatty liver disease (NAFLD)?

Dysregulation of FGF19 is implicated in metabolic diseases, including obesity and NAFLD. Altered FGF19 signaling may contribute to aberrant bile acid and lipid metabolism in these conditions.