Active Recombinant Human FGF4 Protein (177 aa)
Cat.No. : | FGF4-413F |
Product Overview : | Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E. coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE. |
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Species : | Human |
Source : | E.coli |
ProteinLength : | 177 |
Description : | Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis. |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | ED50< 0.5 ng/mL, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >2.0 × 10^6 units/mg. |
Molecular Mass : | 19.4 kDa, observed by reducing SDS-PAGE. |
AA Sequence : | MAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL |
Endotoxin : | < 0.2 EU/μg, determined by LAL method. |
Purity : | > 95% by SDS-PAGE and HPLC analyses. |
Storage : | Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : | Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH7.5. |
Reconstitution : | Reconstituted in ddH2O at 100 μg/mL. |
Gene Name | FGF4 fibroblast growth factor 4 [ Homo sapiens ] |
Official Symbol | FGF4 |
Synonyms | FGF4; fibroblast growth factor 4; heparin secretory transforming protein 1, HSTF1; HBGF 4; HST; HST 1; human stomach cancer; transforming factor from FGF related oncogene; K FGF; kaposi sarcoma oncogene; KFGF; transforming protein KS3; FGF-4; HSTF-1; oncogene HST; heparin-binding growth factor 4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; fibroblast growth factor 4 splice isoform; human stomach cancer, transforming factor from FGF-related oncogene; HST-1; HSTF1; K-FGF; HBGF-4; |
Gene ID | 2249 |
mRNA Refseq | NM_002007 |
Protein Refseq | NP_001998 |
MIM | 164980 |
UniProt ID | P08620 |
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