Fgf4-521M
Optional Service: Optional requirements on this protein
Product Overview :
RecombinantMouse Fgf4 protein was expressed in E. coli. Ala30-Leu202.
Description :
Fgf4 is a 25kDa secreted, heparin-binding member of the FGF family. The mouse Fgf4 cDNA encodes202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature proteinwith an FGF homology domain that contains a heparin binding region near the C-terminus.Expression of Fgf4 and its receptors, FGF R1c, 2c, 3c and 4,is spatially and temporally regulated during embryonic development. Itsexpression in the trophoblast inner cell mass promotes expression of FGF R2,and is required for maintenance of the trophectoderm and primitive endoderm.
Source :
E. coli
Form :
Lyophilizedfrom a 0.2 um filtered solution in MOPS, Ammonium Sulfate, EDTA, DTT with BSAas a carrier protein.
N-terminalSequence :
Ala30
MolecularWeight :
19.0 kDa
Activity :
Measuredin a cell proliferation assay using NR6R3T3 mouse fibroblasts. The ED50 forthis effect is typically 0.75-3.75 ng/mL.
Endotoxin Level :
<1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE :
19 kDa,reducing conditions.
Purity :
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
Reconstituteat 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Storage :
Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.