Active Recombinant Human GPC3 Protein (Gln 25 - His 559), Fc-tagged, low endotoxin

Cat.No. : GPC3-337H
Product Overview : Recombinant Human GPC3 Protein, Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # NP_004475). Predicted N-terminus: Gln25, Ser359, Val483. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. GP3-H5258 may be cleaved into secrete GPC3, short propeptide (aa 483-559 predicted) & Fc tag, long propeptide (aa 359-559 predicted) & Fc tag. The protein migrates as 100 kDa (secrete GPC3 & propeptide & Fc tag), 58 kDa (long propeptide & Fc tag), and 40 kDa (short propeptide & Fc tag) on a SDS-PAGE gel under reducing (R) condition.
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Source : HEK293
Species : Human
Tag : Fc
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human FGF basic, Tag Free at 2 μg/mL (100 μL/well)can bind Recombinant Human GPC3 Protein, Fc-tagged, low endotoxin with a linear range of 4-63 ng/mL (QC tested).
Recombinant Human GPC3 Protein, Fc-tagged, low endotoxin at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.8-6 ng/mL (Routinely tested).
Molecular Mass : 87.4 kDa
Protein length : Gln 25 - His 559
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
SDS-PAGE:
Gene Name : GPC3 glypican 3 [ Homo sapiens ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; SDYS; glypican-3; DGSX; OCI 5; SGB; SGBS; SGBS1; MXR7; OCI-5; GTR2-2;
Gene ID : 2719
mRNA Refseq : NM_001164617
Protein Refseq : NP_001158089
MIM : 300037
UniProt ID : P51654

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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