Recombinant Mouse GPC3 cell lysate

Cat.No. : GPC3-001MCL
Product Overview : Mouse Glypican-3 / GPC3 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.
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Source : Baculovirus-Insect cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : Gpc3 glypican 3 [ Mus musculus ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; glypican-3; OCI-5;
Gene ID : 14734
mRNA Refseq : NM_016697
Protein Refseq : NP_057906
Function : heparan sulfate proteoglycan binding; peptidase inhibitor activity; peptidyl-dipeptidase inhibitor activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Reviews
08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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