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Recombinant Human GPC3 Protein, His-Avi-tagged, Biotinylated

Cat.No.: GPC3-2247H
Product Overview: Biotinylated Human Glypican 3, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source: HEK293
Species: Human
Tag : His-Avi
Predicted N Terminal: Gln 25, Ser 359
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Human FGF basic, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag with a linear range of 1-16 ug/mL. Immobilized Anti-Human GPC3 Mab at 2 ug/mL (100 uL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag with a linear range of 0.4-6 ug/mL.
Molecular Mass: The protein has a calculated MW of 64.5 kDa. The protein migrates as 42 kDa(caused by a furin cleavage site) and 70-250 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >85% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: GPC3 glypican 3 [ Homo sapiens ]
Official Symbol: GPC3
Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2;
Gene ID: 2719
mRNA Refseq: NM_001164617
Protein Refseq: NP_001158089
MIM: 300037
UniProt ID: P51654

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