GPC3-2247H
Optional Service: Optional requirements on this protein
Product Overview :
Biotinylated Human Glypican 3, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Source :
HEK293
Species :
Human
Tag :
His-Avi
Predicted N Terminal :
Gln 25, Ser 359
Form :
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity :
Immobilized Human FGF basic, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag with a linear range of 1-16 ug/mL. Immobilized Anti-Human GPC3 Mab at 2 ug/mL (100 uL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag with a linear range of 0.4-6 ug/mL.
Molecular Mass :
The protein has a calculated MW of 64.5 kDa. The protein migrates as 42 kDa(caused by a furin cleavage site) and 70-250 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin :
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity :
>85% as determined by SDS-PAGE.
Characteristic :
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability :
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage :
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.