Active Recombinant Human IGF1 Protein, Pre-aliquoted

• Cat.No.: IGF1-21H
• Product Overview: Recombinant Human IGF1 Protein, Gly49-Ala118, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.3-1.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass:7.6 kDa
  SDS-PAGE: 7 kDa, reducing conditions
• Protein length: Gly49-Ala118
• N-terminal Sequence Analysis: Gly49
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 200 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: IGF1 insulin like growth factor 1 [ Homo sapiens (human) ]
• Official Symbol: IGF1
• Synonyms: IGF1; insulin like growth factor 1; IGF; MGF; IGFI; IGF-I; insulin-like growth factor I; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor IB; mechano growth factor; somatomedin-C
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 147440
• UniProt ID: P05019