Active Recombinant Human SRC, GST-tagged

• Cat.No.: SRC-575H
• Product Overview: Recombinant Human SRC (Accession # NM_005417) , fused with an N-terminal GST tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
• Bio-activity: The specific activity of Src was determined to be 100 nmol/min/mg using a poly (Glu:Tyr, 4:1) synthetic peptide substrate
• Molecular Mass: In SDS-PAGE migrates as 83 kDa.
• Purity: The purity was determined to be >95% by densitometry
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: SRC
• Synonyms: SRC; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); SRC1, v src avian sarcoma (Schmidt Ruppin A 2) viral oncogene homolog; proto-oncogene tyrosine-protein kinase Src; ASV; c src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; SRC1; c-SRC; p60-Src
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931
• Chromosome Location: 20q12-q13

Reviews

01/01/2022

Top-notch protein purification service, essential for our research.

08/29/2019

Reliable mass spectrometry analysis, pivotal for our proteomics work.

03/02/2019

Swift and reliable protein sequencing, indispensable for our research success.

Q&As

How do alterations in SRC expression or activity affect the development of drug resistance in tumors?

Changes in SRC expression or kinase activity can lead to drug resistance in cancer treatment.

How does SRC (proto-oncogene tyrosine-protein kinase Src) regulate cell proliferation and survival?

SRC regulates cell proliferation and survival through its kinase activity, influencing various signaling pathways.

How does SRC contribute to the regulation of bone remodeling and osteoclast activity?

SRC is involved in bone remodeling, regulating osteoclast activity and bone resorption.

What role does SRC play in cancer development and progression, particularly in metastasis?

SRC plays a crucial role in cancer progression, particularly in enhancing metastatic potential of tumor cells.

How does SRC influence cellular signaling pathways, such as the Ras/MAPK and PI3K/Akt pathways?

SRC influences key cellular signaling pathways like Ras/MAPK and PI3K/Akt, affecting cell growth and survival.

What are the mechanisms by which SRC modulates cellular adhesion and migration?

SRC modulates cellular adhesion and migration, playing a role in tissue repair and cancer metastasis.

What is the impact of SRC inhibition on targeted cancer therapies?

SRC inhibition is a target in cancer therapy, potentially reducing tumor growth and metastasis.