Active Recombinant Mouse CD274 protein, Fc-tagged

• Cat.No.: CD274-0624M
• Product Overview: Recombinant Mouse CD274 protein(Phe19-Thr238), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Mouse PD-L1, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag with a linear range of 0.156-2.5 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.4 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe19-Thr238
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693