Active Recombinant Mouse Fgf21 Protein (182 aa)

• Cat.No.: Fgf21-414F
• Product Overview: Recombinant Mouse Fibroblast Growth Factor-21(FGF-21) produced in E. coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rmFGF-21 has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Description: Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 0.5 μg/mL,measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 μg/mL mouse Klotho and 10 μg/mL heparin, corresponding to a specific activity of > 2 × 10^3 units/mg.
• Molecular Mass: 19.9 kDa, observed by reducing SDS-PAGE.
• Protein Length: 182
• AA Sequence: AYPIPDSSPLLQFGGQVRQRYLYTD DDQDTEAHLEIREDGTVVGAAHRSP ESLLELKALKPGVIQILGVKASRFL CQQPDGALYGSPHFDPEACSFRELL LEDGYNVYQSEAHGLPLRLPQKDSP NQDATSWGPVRFLPMPGLLHEPQDQ AGFLPPEPPDVGSSDPLSMVEPLQG RSPSYAS
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 97% as analyzed by SDS-PAGE& HPLC.
• Storage: Lyophilized recombinant Mouse Fibroblast Growth Factor-21(FGF-21), remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Mouse FGF-21 should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus ]
• Official Symbol: Fgf21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397
• UniProt ID: Q9JJN1

Reviews

01/04/2022

The manufacturer's history of producing high-quality products contributed to our trust in this protein.

06/27/2021

Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

03/03/2016

The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

Q&As

How is FGF21 expression regulated in adipose tissue?

FGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.

What are the effects of FGF21 on lipid metabolism?

FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.

Can FGF21 be a biomarker for metabolic health?

FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.

Is FGF21 involved in the regulation of insulin sensitivity?

Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.

Does FGF21 have roles in energy expenditure?

Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.

Are there gender-specific differences in FGF21 levels?

Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.

What signaling pathways does FGF21 activate?

FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.