Recombinant Rat Fgf21 Protein, His-tagged

• Cat.No.: Fgf21-1491R
• Product Overview: Recombinant Rat Fgf21 Protein (Ala29-Ala207) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Involved in several processes, including cellular response to glucagon stimulus; endothelial cell apoptotic process; and negative regulation of cell death. Located in extracellular space. Orthologous to human FGF21 (fibroblast growth factor 21).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.0 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Ala29-Ala207
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Fgf21
• Synonyms: FGF21; fibroblast growth factor 21
• Gene ID: 170580
• mRNA Refseq: NM_130752
• Protein Refseq: NP_570108
• UniProt ID: Q9JJN1

Reviews

01/04/2022

The manufacturer's history of producing high-quality products contributed to our trust in this protein.

06/27/2021

Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

03/03/2016

The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

Q&As

How is FGF21 expression regulated in adipose tissue?

FGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.

What are the effects of FGF21 on lipid metabolism?

FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.

Can FGF21 be a biomarker for metabolic health?

FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.

Is FGF21 involved in the regulation of insulin sensitivity?

Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.

Does FGF21 have roles in energy expenditure?

Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.

Are there gender-specific differences in FGF21 levels?

Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.

What signaling pathways does FGF21 activate?

FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.