Recombinant Pig IL2 protein, His-tagged

Cat.No. : IL2-1027P
Product Overview : Recombinant Pig IL2 protein(Ala21~Thr154), fused with N-terminal His tag, was expressed in E.coli.
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Species : Pig
Source : E.coli
Tag : His
Protein Length : 21-154 aa
Form : PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Bio-activity : IL-2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response.Therefore, in order to detect the bioactivity of recombinant pig IL-2, spleen single suspensions were prepared, activated with conA (final concentration 3 ug/ml). Cells were collected after 72h and washed with hanks.Then mouse splenic lymphocytes were were seeded into triplicate wells of 96-well plates at a density of 10,000 cells/well with or without the addition of various concentrations of recombinant pig IL-2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10 µl of CCK-8 solution was added to each well of the plate, the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Splenic lymphocytes cells after incubation with IL-2 for 96h observed by inverted microscope was shown. The dose-effect curve of recombinant pig IL-2 was obvious that recombinant pig IL-2 significantly promoted cell proliferation of Splenic lymphocytes cells. The EC50 for this effect is typically 0.011-0.021 ug/ml.
Molecular Mass : 18.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : >90%
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name IL2 interleukin 2 [ Sus scrofa ]
Official Symbol IL2
Synonyms IL2; interleukin 2; interleukin-2; T-cell growth factor; IL-2; TCGF; POIL2;
Gene ID 396868
mRNA Refseq NM_213861
Protein Refseq NP_999026

Not For Human Consumption!

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