| Species : |
Pig |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
21-154 aa |
| Form : |
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
| Bio-activity : |
IL-2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response.Therefore, in order to detect the bioactivity of recombinant pig IL-2, spleen single suspensions were prepared, activated with conA (final concentration 3 ug/ml). Cells were collected after 72h and washed with hanks.Then mouse splenic lymphocytes were were seeded into triplicate wells of 96-well plates at a density of 10,000 cells/well with or without the addition of various concentrations of recombinant pig IL-2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10 µl of CCK-8 solution was added to each well of the plate, the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Splenic lymphocytes cells after incubation with IL-2 for 96h observed by inverted microscope was shown. The dose-effect curve of recombinant pig IL-2 was obvious that recombinant pig IL-2 significantly promoted cell proliferation of Splenic lymphocytes cells. The EC50 for this effect is typically 0.011-0.021 ug/ml. |
| Molecular Mass : |
18.9kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
>90% |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
