Active Recombinant Human IL2 protein, His-tagged
Cat.No. : | IL2-473H |
Product Overview : | Active Recombinant Human IL2 protein(P60568)(21~153 aa), fused with N-terminal His tag, was expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | 21-153 aa |
Form : | Lyophilized from sterile PBS, pH7.6, containing 5% trehalose. |
Bio-activity : | IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10µL of CCK-8 solution was added to each well of the plate, the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope was shown. |
Molecular Mass : | 17.0kDa |
Purity : | > 95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | IL2 interleukin 2 [ Homo sapiens ] |
Official Symbol | IL2 |
Synonyms | IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine; |
Gene ID | 3558 |
mRNA Refseq | NM_000586 |
Protein Refseq | NP_000577 |
MIM | 147680 |
UniProt ID | P60568 |
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Not For Human Consumption!
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