Active Recombinant Human IL2 protein, His-tagged

Cat.No. : IL2-473H
Product Overview : Active Recombinant Human IL2 protein(P60568)(21~153 aa), fused with N-terminal His tag, was expressed in HEK293.
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Species : Human
Source : HEK293
Tag : His
Protein Length : 21-153 aa
Form : Lyophilized from sterile PBS, pH7.6, containing 5% trehalose.
Bio-activity : IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10µL of CCK-8 solution was added to each well of the plate, the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope was shown.
Molecular Mass : 17.0kDa
Purity : > 95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name IL2 interleukin 2 [ Homo sapiens ]
Official Symbol IL2
Synonyms IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine;
Gene ID 3558
mRNA Refseq NM_000586
Protein Refseq NP_000577
MIM 147680
UniProt ID P60568

Not For Human Consumption!

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