Description : |
Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. |
Source : |
E. coli |
Species : |
Pig |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
ED50 < 1 ng/mL, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0 × 10^6 IU/mg. |
Molecular Mass : |
17.7 kDa, observed by reducing SDS-PAGE. |
Protein Length : |
154 |
AA Sequence : |
MANVQSMECKLQDKDHKSLVLAGPH MLKALHLLTGDLKREVVFCMSFVQG DDSNNKIPVTLGIKGKNLYLSCVMK DNTPTLQLEDIDPKRYPKRDMEKRF VFYKTEIKNRVEFESALYPNWYIST SQAEQKPVFLGNSKGRQDITDFTME VLSP |
Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
Purity : |
> 95% as analyzed by SDS-PAGE. |
Storage : |
Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |