Active Recombinant Rat Il1b protein, His-tagged

• Cat.No.: Il1b-1019R
• Product Overview: Recombinant Rat Il1b protein(Val117-Ser268), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 117-268 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Recombinant Rat IL-1 beta Protein stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.68-2.72 ng/mL, corresponding to a specific activity of 3.68×10^5~1. 47×10^6 units/mg.
• Molecular Mass: 18.16 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Rattus norvegicus ]
• Official Symbol: Il1b
• Synonyms: IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta
• Gene ID: 24494
• mRNA Refseq: NM_031512
• Protein Refseq: NP_113700