Active Recombinant Rat Chemokine (C-X3-C motif) Ligand 1

Cat.No. : Cx3cl1-460R
Product Overview : Recombinant Rat Cx3cl1protein was expressed in Sf21. Gln25-Thr334.
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Cat. No. : Cx3cl1-460R
Description : Cx3cl1, also named neurotactin, is a member of the delta chemokinesubfamily that contains a novel C-X3-C motif. Unlike other known chemokines, Cx3cl1 is a type 1 membrane protein containing a chemokine domaintethered on a long mucinlike stalk. Rat Cx3cl1cDNA encodes a 393 amino acid (aa) residue precursor protein with two alternative(21 aa or 24 aa residue) putative signal peptides, a 76 aa residue globular chemokinedomain, a 238 aa residue stalk region rich in Gly, Pro, Ser and Thr and containingdegenerate mucinlike repeats, a 19 aa residue transmembrane segment and a 36 aaresidue cytoplasmic domain.
Source : Spodoptera frugiperda,Sf 21 (baculovirus)
Form : Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence : No results obtained:Gln25 predicted
Molecular Weight : 33 kDa
Activity : Measuredby its ability to chemoattract mouse Cx3cl1 transfected BaF3 mouse proB cells. The ED50 forthis effect is typically 0.02-0.08 ng/mL.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
Purity : >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstituteat 100 μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage : Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
Gene Name : Cx3cl1 chemokine(C-X3-C motif) ligand 1 [ Rattus norvegicus ]
Official Symbol : Cx3cl1
Synonyms : Cx3cl1; chemokine(C-X3-C motif) ligand 1; Cx3c; Scyd1; fractalkine; neurotactin; C-X3-C motif chemokine1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small induciblecytokine subfamily D, 1; Acc1; Fkn
Gene ID : 89808
mRNA Refseq : NM_134455
Protein Refseq : NP_604450
UniProt ID : O55145
Chromosome Location : 19p12
Pathway : Chemokine receptors bindchemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-like receptors);Cytokine-cytokine receptor interaction; GPCR ligand binding; Peptide ligand-bindingreceptors; Signal Transduction; Signaling by GPCR
Function : chemokine activity

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