Active Native Human Coagulation Factor XII (Hageman factor)

• Cat.No.: F12-5397H
• Product Overview: HumanFactor beta-XIIa is a single chain polypeptide activated from homogeneous Human F12 by an autoactivation process with dextran sulfate.

Specification

• Description: Human F12is generated in vivo by kallikrein cleavage of Factor alpha-XIIa and containsthe catalytic light chain and the C-terminal peptide of the heavy chain of Factoralpha-XIIa. Human Factor beta-XIIa is activated from homogeneous Human FactorXII by an autoactivation process with Dextran Sulfate followed byrepurification to isolate Factor alpha-XIIa and Factor beta-XIIa. Completeactivation is observed on SDSPAGE.
• Source: Human plasma
• Species: Human
• Form: Frozen liquidcontaining 4 mM Sodium acetate Buffer + 0.15 M NaCl, pH 5.3.
• Bio-activity: Determined byClotting Assay.
• Molecular Mass: 30 kDa
• Endotoxin: < 0.1 ng/μg ofprotein.>
• Purity: ≥95% by SDS-PAGEanalysis.
• Storage: Store at-80°C. Stable for 3 years from delivery. Avoid repeated freeze-thaw cycles.
• Concentration: 2.0 mg/ml

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Homo sapiens ]
• Official Symbol: F12
• Synonyms: F12; coagulation factor XII (Hageman factor); coagulation factor XII; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; HAF; HAE3; HAEX
• Gene ID: 2161
• mRNA Refseq: NM_000505
• Protein Refseq: NP_000496
• MIM: 610619
• UniProt ID: P00748
• Chromosome Location: 5q33-qter
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem
• Function: binding; misfolded protein binding; peptidase activity; protein binding; serine-type endopeptidase activity