Active Recombinant Mouse Left Right Determination Factor 1

• Cat.No.: Lefty1-579M
• Product Overview: Recombinant Mouse PDGF-BB is a non-glycosylated polypeptide consisting of two PDGF-B chains of 109 amino acids each. The final protein is a disulfide-linked homodimer of 218 amino acids with a molecular weight of 24.6 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Recombinant Mouse Platelet Derived Growth Factor BB is part of a family of PDGFs incuding AA, AB and BB. All of them are disulfide-linked homodimers and play an important role in the mitogenic activity for connective tissue, blood, bone and cartilage cells.
• Form: Lyophilized from 10 mM NaCitrate, pH 3.0.
• Purity: Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE. Analytical HPLC.
• Bio-activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of <1.6 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE. Analytical HPLC
• Protein Content determined by: UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRPVQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Lefty1 left right determination factor 1 [ Mus musculus ]
• Official Symbol: Lefty1
• Synonyms: Lefty1; left right determination factor 1; left-right determination factor 1; Left-right determination factor B; LEFTYB; Protein lefty-1; Protein lefty-B; left-right determination, factor B; Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052; TGF-beta-4; OTTMUSP00000023876; transforming growth factor beta-4; stimulated by retinoic acid gene 3 protein
• Gene ID: 13590
• mRNA Refseq: NM_010094
• Protein Refseq: NP_034224
• UniProt ID: Q64280
• Chromosome Location: 1; 1 F
• Pathway: Formation of Platelet plug; Hemostasis; Platelet Activation; Platelet degranulation; PluriNetWork; Response to elevated platelet cytosolic Ca2+; TGF-beta signaling pathway
• Function: cytokine activity; growth factor activity; transforming growth factor beta receptor binding

Citation

Recombinant frizzled1 protein attenuated cardiac hypertrophy after myocardial infarction via the canonical Wnt signaling pathway

Journal: Oncotarget    PubMed ID: 29423029    Data: 2018/1/9

Authors: Jingjing Fan, Lin Qiu, Ning Zhou

Article Snippet:Five weeks old female A/J mice were used.Five weeks old female A/J mice were used.. The mice were subcutaneously injected with 100 ul emulsion of 150 ug mouse RFP (Creative BioMart, USA) or supplemented Complete Freund’s Adjuvant (CFA) at the first and 7th day of the study.. 3-weeks after the first injection of RFP, the mice were subjected to the LAD coronary ligation or sham operation as described previously [ ].3-weeks after the first injection of RFP, the mice were subjected to the LAD coronary ligation or sham operation as described previously [ ].