| Cat. No.: | Lefty1-579M |
| Product Overview: | Recombinant Mouse PDGF-BB is a non-glycosylated polypeptide consisting of two PDGF-B chains of 109 amino acids each. The final protein is a disulfide-linked homodimer of 218 amino acids with a molecular weight of 24.6 kDa. |
| Description: | Recombinant Mouse Platelet Derived Growth Factor BB is part of a family of PDGFs incuding AA, AB and BB. All of them are disulfide-linked homodimers and play an important role in the mitogenic activity for connective tissue, blood, bone and cartilage cells. |
| Species: | Mouse |
| Source: | E.coli |
| Form: | Lyophilized from 10 mM NaCitrate, pH 3.0. |
| Purity: | Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE. Analytical HPLC. |
| Bio-activity: | The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of <1.6 ng/ml. |
| Physical Appearance: | Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility: | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 98% as determined by: | Reducing and non-reducing SDS-PAGE. Analytical HPLC |
| Protein Content determined by: | UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level: | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence: | MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRPVQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT |
| Storage: | Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
| Gene Name: | Lefty1 left right determination factor 1 [ Mus musculus ] |
| Official Symbol: | Lefty1 |
| Synonyms: | Lefty1; left right determination factor 1; left-right determination factor 1; Left-right determination factor B; LEFTYB; Protein lefty-1; Protein lefty-B; left-right determination, factor B; Ebaf; Leftb; Stra3; Tgfb4; lefty; lefty-1; AI450052; TGF-beta-4; OTTMUSP00000023876; transforming growth factor beta-4; stimulated by retinoic acid gene 3 protein |
| Gene ID: | 13590 |
| mRNA Refseq: | NM_010094 |
| Protein Refseq: | NP_034224 |
| UniProt ID: | Q64280 |
| Chromosome Location: | 1; 1 F |
| Pathway: | Formation of Platelet plug; Hemostasis; Platelet Activation; Platelet degranulation; PluriNetWork; Response to elevated platelet cytosolic Ca2+; TGF-beta signaling pathway |
| Function: | cytokine activity; growth factor activity; transforming growth factor beta receptor binding |
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