||Recombinant Human Fibroblast Growth Factor -19 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23 kDa. The amino acid sequence of the recombinant human FGF19 is 100% homologous to the amino acid sequence of the human FGF19 without signal sequence and contains his-tag at N-terminal. The FGF-19 is purified by proprietary chromatographic techniques.
||The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. FGF-19, has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
|Amino Acid Sequence:
||MRGSHHHHHH GMASLAFSDA GPHVHYGWGD PIRLRHLYTS GPHGLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK
||Sterile Filtered white lyophilized powder.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (c) Analysis by SDS-PAGE.
||Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 20 mM TRIS, 20 mM NaCl, pH 7.5.
||It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-19 Human Recombinant sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.