||Recombinant Human E2F1 fussed with MYC/DDK tag at C-terminal was expressed in HEK293 cells.
||The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
||>80% as determined by SDS-PAGE and Coomassie blue staining
||>50 ug/mL as determined by microplate BCA method