Active Recombinant Mouse Adam10 Protein, His-tagged

• Cat.No.: Adam10-961M
• Product Overview: Recombinant Mouse Adam10 (Accession # O35598) Thr215-Glu673, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Thr215
• Form: Lyophilized from a 0.2 µm filtered solution in MES, NaCl, CaCl2, ZnCl2, and Brij-35.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2.The specific activity is >5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Adam10, His-tagged has a calculated MW of 52 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Thr215-Glu673
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Adam10 a disintegrin and metallopeptidase domain 10 [ Mus musculus ]
• Official Symbol: Adam10
• Synonyms: ADAM10; a disintegrin and metallopeptidase domain 10; disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloprotease domain (ADAM) 10; kuz; MADM; kuzbanian; 1700031C13Rik
• Gene ID: 11487
• mRNA Refseq: NM_007399
• Protein Refseq: NP_031425