Recombinant Mouse Adam10, His-tagged

• Cat.No.: Adam10-1927M
• Product Overview: Recombinant Mouse Adam10 (Thr215-Glu673), fused with a C terminal 10 His tag, was expressed in Sf 21 Cells.

Specification

• Species: Mouse
• Source: Sf21 Cells
• Tag: His
• Protein Length: 215-673 a.a.
• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. Sheddase, a generic name for the ADAM metallopeptidase, functions primarily to cleave membrane proteins at the cellular surface. Once cleaved, the sheddases release soluble ectodomains with an altered loca-tion and function.
• Molecular Mass: The predicted molecular mass of this protein is 52 KDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Purity: >90%, by SDSPAGE
• Bio-Activity: Measured by its ability to cleave a fluorogenic peptide substrate McaPLAQAVDpaRSSSR-NH2. The specific activity is >5 pmol/min/μg
• Form: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, ZnCl2, and Brij35.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 25 mM Tris, pH 7.5.
• Storage: Store at -200C ~ -700C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: Adam10

Gene Information

• Gene Name: Adam10 a disintegrin and metallopeptidase domain 10 [ Mus musculus ]
• Synonyms: Adam10; a disintegrin and metallopeptidase domain 10; kuz; MADM; kuzbanian; 1700031C13Rik; Adam10
• Gene ID: 11487
• mRNA Refseq: NM_007399
• Protein Refseq: NP_031425
• UniProt ID: O35598
• Chromosome Location: 9 D; 9 41.0 cM
• Pathway: Alzheimer"s disease
• Function: SH2 domain binding; SH3 domain binding; integrin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; protein homodimerization activity; protein kinase binding; zinc ion binding; metalloendopeptidase activity