Active Recombinant Mouse GDF11

• Cat.No.: GDF11-1079M
• Product Overview: Recombinant Mouse GDF11 (Accession # Q9Z1W4) Asn299-Ser407, fused with an N-terminal Met, was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50
• Molecular Mass: Recombinant Mouse GDF11, Met-tagged has a calculated MW of 12.6 kDa. In SDS-PAGE migrates as 13 kDa, reducing conditions.
• Protein length: Asn299-Ser407
• Purity: Assessed by mass spectrometry, and is >95% pure by SDS-PAGE under reducing conditions visualized by silver stain (see below).
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Gdf11 growth differentiation factor 11 [ Mus musculus ]
• Official Symbol: GDF11
• Synonyms: GDF11; growth differentiation factor 11; growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11; Bmp11; BMP-11
• Gene ID: 14561
• mRNA Refseq: NM_010272
• Protein Refseq: NP_034402