Recombinant Human F2 Protein, His-tagged

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Recombinant Human F2 Protein, His-tagged

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Cat.No. : F2-1103H
Product Overview : Recombinant Human F2 (Accession # P00734) Met1-Glu622, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Thr328, Ile364 & Val515
Form : Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human F2, His-tagged has a calculated MW of 4 kDa (light chain A), 31 kDa (heavy chain B), 14 kDa (C-terminal fragment). In SDS-PAGE migrates as 12 kDa, 21 kDa and 23 kDa, reducing conditions.
Protein length : Met1-Glu622
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : F2 coagulation factor II (thrombin) [ Homo sapiens ]
Official Symbol : F2
Synonyms : F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1;
Gene ID : 2147
mRNA Refseq : NM_000506
Protein Refseq : NP_000497
MIM : 176930
UniProt ID : P00734
Chromosome Location : 11p11-q12
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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