Recombinant Human F2 Protein, His-tagged F2-1103H

Recombinant Human F2 Protein, His-tagged


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Recombinant Human F2 Protein, His-tagged

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Cat.No. : F2-1103H
Product Overview : Recombinant Human F2 (Accession # P00734) Met1-Glu622, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Human
Tag : His
Predicted N Terminal : Thr328, Ile364 & Val515
Form : Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human F2, His-tagged has a calculated MW of 4 kDa (light chain A), 31 kDa (heavy chain B), 14 kDa (C-terminal fragment). In SDS-PAGE migrates as 12 kDa, 21 kDa and 23 kDa, reducing conditions.
Protein length : Met1-Glu622
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : F2 coagulation factor II (thrombin) [ Homo sapiens ]
Official Symbol : F2
Synonyms : F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1;
Gene ID : 2147
mRNA Refseq : NM_000506
Protein Refseq : NP_000497
MIM : 176930
UniProt ID : P00734
Chromosome Location : 11p11-q12

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