"F2" Related Products

Recombinant Human F2 Protein, His-tagged

Cat.No.: F2-1103H
Product Overview: Recombinant Human F2 (Accession # P00734) Met1-Glu622, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Tag: His
Predicted N Terminal: Thr328, Ile364 & Val515
Form: Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human F2, His-tagged has a calculated MW of 4 kDa (light chain A), 31 kDa (heavy chain B), 14 kDa (C-terminal fragment). In SDS-PAGE migrates as 12 kDa, 21 kDa and 23 kDa, reducing conditions.
Protein length: Met1-Glu622
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: F2 coagulation factor II (thrombin) [ Homo sapiens ]
Official Symbol: F2
Synonyms: F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1;
Gene ID: 2147
mRNA Refseq: NM_000506
Protein Refseq: NP_000497
MIM: 176930
UniProt ID: P00734
Chromosome Location: 11p11-q12

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