Recombinant Mouse Prf1 protein, His/GST-tagged Prf1-25M

Recombinant Mouse Prf1 protein, His/GST-tagged

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Recombinant Mouse Prf1 protein, His/GST-tagged

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Cat.No. : Prf1-25M
Product Overview : Recombinant Mouse Prf1(Val40-Lys355) fused with His/GST tag at N-terminal was expressed in E. coli.
Description : perforin 1 (pore forming protein) played an important role in many biological functions.
Source : E. coli
Species : Mouse
Tag : His/GST
Form : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Molecular Mass : 67.2kDa
AA Sequence : MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- V WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPK
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : SDS-PAGE; WB; ELISA; IP.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : Prf1 perforin 1 (pore forming protein) [ Mus musculus ]
Official Symbol : Prf1
Synonyms : PRF1; perforin 1 (pore forming protein); perforin-1; P1; cytolysin; pore forming protein; lymphocyte pore-forming protein; Pfn; Pfp; Prf-1;
Gene ID : 18646
mRNA Refseq : NM_011073
Protein Refseq : NP_035203
UniProt ID : P10820
Chromosome Location : 10 B4; 10 32.18 cM
Pathway : Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Apoptosis, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Graft-versus-host disease, organism-specific biosystem; Graft-versus-host disease, conserved biosystem;
Function : calcium ion binding; metal ion binding; wide pore channel activity; wide pore channel activity;

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