Recombinant Mouse Prf1 protein, His/GST-tagged

• Cat.No.: Prf1-25M
• Product Overview: Recombinant Mouse Prf1(Val40-Lys355) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: perforin 1 (pore forming protein) played an important role in many biological functions.
• Source: E. coli
• Species: Mouse
• Tag: His/GST
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 67.2kDa
• AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- V WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPK
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Prf1 perforin 1 (pore forming protein) [ Mus musculus ]
• Official Symbol: Prf1
• Synonyms: PRF1; perforin 1 (pore forming protein); perforin-1; P1; cytolysin; pore forming protein; lymphocyte pore-forming protein; Pfn; Pfp; Prf-1
• Gene ID: 18646
• mRNA Refseq: NM_011073
• Protein Refseq: NP_035203
• UniProt ID: P10820
• Chromosome Location: 10 B4; 10 32.18 cM
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Apoptosis, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Graft-versus-host disease, organism-specific biosystem; Graft-versus-host disease, conserved biosystem
• Function: calcium ion binding; metal ion binding; wide pore channel activity; wide pore channel activity