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Recombinant Human IL12A

Cat.No. : IL12A-112H
Product Overview : Recombinant Human interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) sequence (containing the signal peptide and the mature IL-12 alpha chain sequence, and the signal peptide and the mature IL-12 beta chain sequence) was expressed in modifiedhuman 293 cells.
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Cat. No. : IL12A-112H
Description : Human interleukin 12 (IL-12, IL12) is a pro-inflammatory cytokine that consists of a heterodimer comprising two disulfide-linked subunits, the alpha chain (the p35 subunit, IL-12A) and the beta chain (the p40 subunit, IL-12B).
Source : Human 293 cells.
Amino Acid Sequence : HGTELPSPPSVWFEA EFFHHILHWTPIPNQSES TCYEVALLRYGIESW NSISNCSQ TLSYDLT AVTL DLYHSNG YRARVRAV DGSRHSNWTV TNTRFSVDEVT LT V G SVNLEIH NGFIL GKIQLPR PK MAPANDTY ESIFSHF REYEIAIR KVP GN F T F THKKVK HENFSLLTSGEVGE FCV QVK PSV ASRS NKGMWSK EE CIS LTRQY FTGIPKVDKKV EPK SC DKT HTC PPCPA PELLGGPSV FLFP PKP KDT LM IS RTP EVT CVVVDVS HED PEVKF NWYVDGV EVHNAKTKPREEQYNST YRV VSV L TVLHQ DWLNG EYKCRVS NKALPAPI EKTISKAK GQPREPQ VYTLPPSR DELTK NQVSL TCLVK GFYPSD IAVEWE SNGQPE NNYKTTP PVLDSDGSFFLY SKL TV DKSRWQ QGNVFSCSVMH EALHNHYT QKSLSLSPGK.
Molecular Mass : IL-12A is a disulfide-linked heterodimeric glycoprotein, consisting of an alpha and beta chain.IL-12A migrates as multiple bands between 30 and 45 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. The IL-12A alpha chain migrates as bands between 30 and 35 kDa. This compares with the unmodified IL-12 alpha chain that has a predicted molecular mass of 22.5 kDa. The IL-12A beta chain migrates as bands between 35 and 45 kDa. This compares with the unmodified IL-12 beta chain that has a predicted molecular mass of 34.7 kDa.
PI : IL-12 separates into a number of isoforms with a pI between 3.5 and 9.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. IL-12 alpha chain separates into multiple isoforms with a pI between 3.5 and 9.5. This compares with the unmodified IL-12 alpha chain that has a predicted pI of 6.1. The Symansis IL-12 beta chain separates into multiple isoforms with a pI between 5.0 and 9.0. This compares with the unmodified IL-12 beta chain that has a predicted pI of 5.4.
% Carbohydrate : Purified IL-12 alpha chain consists of 15-35% carbohydrate by weight. purified IL-12 beta chain consists of 0-25% carbohydrate by weight.
Glycosylation : IL-12 has N- and O-glycosylation.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Gene Name : interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Synonyms : IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain;Cytotoxic lymphocyte maturation factor 35 kDa subunit
Gene ID : 3592
mRNA Refseq : NM_000822
Protein Refseq : NP_000873
MIM : 161560
UniProt ID : P29459
Chromosome Location : 3q25.33-q26
Pathway : Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
Function : cytokine activity; growth factor activity; interleukin-12 receptor binding ; interleukin-12 beta subunit binding ; interleukin-27 binding protein binding; protein heterodimerization activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do mass spectrometry-based techniques reveal post-translational modifications on IL12A? 08/18/2022

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models? 06/12/2022

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners? 06/26/2021

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture? 12/17/2020

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors? 10/25/2020

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells? 05/19/2019

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors? 06/05/2018

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function? 03/08/2018

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains? 11/23/2017

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms? 02/16/2017

Bioluminescence imaging tracks IL12A expression dynamics in vivo.

Customer Reviews (3)

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Reviews
01/14/2021

    Suitable for co-immunoprecipitation.

    12/21/2020

      Consistent in bioactivity.

      11/16/2020

        Good for receptor binding studies.

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