Recombinant Human Interleukin-12 IL12-21H

Recombinant Human Interleukin-12


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Recombinant Human Interleukin-12

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Cat.No. : IL12-21H Optional Service: Optional requirements on this protein
Product Overview : Recombinant human interleukin 12 is a heterodimeric cytokine composed of covalently disulfide linked a light chain of p35 (P35, IL-12α) and a heavy chain of p40 (P40, IL-12β) with PI 4.5-5.5. Specifically, the heavy chain (P40) is consisted of 306 amino acids, while the light chain (P35) compounds 197 amino acids. It is approximately 75 kDa, containing 503 amino acids, non-glycosylated.
Description : Inerleukin-12 (IL-12), as a potent factor, plays an important role in the networks of immune system. It is a multifunctional cytokine, the properties of which bridge innate and adaptive immunity, acting as a key regulator of cell-mediated immune responses through the induction of T helper 1differentiation. By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. IL-12 not only can facilitate the maturation of antigen presenting cells (APC), especially for dendritic cells (DC), but also can be the third signal to change properties of CD8 T cells and increase generation of effector and memory cells. Treatment with IL-12 can inhibit bacterial, virus and intracellular parasites, produce an effect of anti-tumor, and enhance autoimmunity.
Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses.
Specification : 10μg/bottle. Any other specifications, please feel free to contact us.
Formulation : Sterile filtered through 0.2 micron filter, added the human serum albumin and mannitol and then lyophilized from 10mMPBS, pH=7.2.
Specific Activity : the ED50was determined by the stimulation of IFN-γ production by human peripheral blood mononuclear cells co-stimulated with IL-12 is ≤ 5ng/ml, corresponding to a specific activity of ≥ 8000 units/μg.
Endotoxin : Endotoxin level is less than 1 EU/μg.
Reconstitution : Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 day or -20°C for future use.
Storage : The lyophilized is stable for 3 months at room temperature, can be stored for 2 years at 4°C , but best stored at -20°C . Reconstituted IL-12 should be stored in working aliquots at -20°C .
Crystal structure of human IL-12 .FromPDB1F455 :

Gene Name : IL12A
Synonyms : interleukin12A(natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); P35; CLMF; NFSK; NKSF1; IL-12A; natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12 p35;IL-12 subunit p35;NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL12A_HUMAN; Interleukin-12 subunit alpha [Precursor];IL-12 subunit p35;Cytotoxic lymphocyte maturation factor 35 kDa subunit;CLMF p35; IL12A
mRNA Refseq : NM_000882
Protein Refseq : NP_000873
MIM : 161560
UniProt ID : P29459
Chromosome Location : 3q25.33-q26
Gene ID : 3593
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus.
Function : Contributes to cytokine activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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