| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Inerleukin-12 (IL-12), as a potent factor, plays an important role in the networks of immune system. It is a multifunctional cytokine, the properties of which bridge innate and adaptive immunity, acting as a key regulator of cell-mediated immune responses through the induction of T helper 1differentiation. By promoting IFN-γ production, proliferation, and cytolytic activity of natural killer and T cells, IL-12 induces cellular immunity. IL-12 not only can facilitate the maturation of antigen presenting cells (APC), especially for dendritic cells (DC), but also can be the third signal to change properties of CD8 T cells and increase generation of effector and memory cells. Treatment with IL-12 can inhibit bacterial, virus and intracellular parasites, produce an effect of anti-tumor, and enhance autoimmunity. |
| Purity : |
Greater than 98% by SDS-PAGE gel and HPLC analyses. |
| Specification : |
10μg/bottle. Any other specifications, please feel free to contact us. |
| Formulation : |
Sterile filtered through 0.2 micron filter, added the human serum albumin and mannitol and then lyophilized from 10mMPBS, pH=7.2. |
| Specific Activity : |
the ED50was determined by the stimulation of IFN-γ production by human peripheral blood mononuclear cells co-stimulated with IL-12 is ≤ 5ng/ml, corresponding to a specific activity of ≥ 8000 units/μg. |
| Endotoxin : |
Endotoxin level is less than 1 EU/μg. |
| Reconstitution : |
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 day or -20°C for future use. |
| Storage : |
The lyophilized is stable for 3 months at room temperature, can be stored for 2 years at 4°C , but best stored at -20°C . Reconstituted IL-12 should be stored in working aliquots at -20°C . |
| Publications : |
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