||Recombinant Human IL1F7 encoding the human IL1F7 Isoform B (Val46–Asp218) was expressed inE.coli. It was recombined with a Hexa-his N-Terminus and purified by Nicolumn.MW=19.3 KD.
||IL-1F7 binds to interleukin-18 receptor (IL-18R) receptor but not to IL-1 receptor. That Could be a new player in the inflammatory and immune responses mediated by the IL-18/IL-18R axis. IL1F7 isoform B were expressed in testis, colon, placenta, lung and lymph node. It was reported that isoform B were expressed in kidney.
||Human IL1F7 isoform B (Val46–Asp218)46 VHTSPKVKNL NPKKFSIHDQ DHKVLVLDSG NLIAVPDKNY IRPEIFFALA SSLSSASAEK GSPILLGVSK GEFCLYCDKD KGQSHPSLQL KKEKLMKLAA QKESARRPFI FYRAQVGSWN MLESAAHPGW FICTSCNCNE PVGVTDKFEN RKHIEFSFQP VCKAEMSPSE VSD 216
||Lyophilized 100μg humanIL1F7 in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
||>95% by SDS gel.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL . Allow to set at least 30 minutes at 4℃, mix well.
||Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.