|Product Overview:||Recombinant Human CSF1, Glu33-Ser190, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is typically 0.5-1.5 ng/mL.
The specific activity of Recombinant Human M-CSF is approximately 1.68 x 10^5 IU/μg, which is calibrated against recombinant human
M-CSF WHO International Standard (NIBSC code: 89/512).
|Molecular Mass:||Predicted Molecular Mass: 18.5 (monomer) kDa
SDS-PAGE: 37 kDa, reducing conditions
|N-terminal Sequence Analysis:||Met-Glu-Glu-Val-Ser-Glu-Try-(Cys)-Ser-His|
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 50-500 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]|
|Synonyms:||CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1;|
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