Active GMP Recombinant Human CSF1 Protein

• Cat.No.: CSF1-110HG
• Product Overview: Recombinant Human CSF1, Glu33-Ser190, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is typically 0.5-1.5 ng/mL.
  The specific activity of Recombinant Human M-CSF is approximately 1.68 x 10^5 IU/μg, which is calibrated against recombinant human
  M-CSF WHO International Standard (NIBSC code: 89/512).
• Molecular Mass: Predicted Molecular Mass: 18.5 (monomer) kDa
  SDS-PAGE: 37 kDa, reducing conditions
• N-terminal Sequence Analysis: Met-Glu-Glu-Val-Ser-Glu-Try-(Cys)-Ser-His
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 50-500 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603