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MMP-9 Fluorimetric Assay Kit

Cat.No. : Kit-3100
Product Overview : The MMP-9 Assay Kit provides a convenient assay for high throughput screening of MMP-9 inducers and inhibitors. It detects MMP-9 activity in a variety of biological samples using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET)5 peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon cleavage into two separate fragments by MMP-9 (Scheme 1), the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. Compared to an EDANS/DABCYL FRET substrate, this 5-FAM/QXL520 substrate provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
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Storage : Store all kit Components at -20centigrade
Protect Components A and B from light and moisture
Components D and E can be stored at 4centigrade for convenience
Size : 100 Assays

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the consequences of dysregulated MMP9 expression in cancer progression? 02/13/2022

Dysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.

What is the impact of MMP9 polymorphisms on enzyme activity? 09/07/2021

Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.

Can you explain the role of MMP9 in tissue remodeling at the molecular level? 01/17/2020

MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.

What is the role of MMP9 in the breakdown of the blood-brain barrier during neurological disorders? 11/14/2019

MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.

How does MMP9 contribute to angiogenesis at the molecular level? 06/02/2018

MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.

Customer Reviews (3)

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Reviews
05/03/2020

    The superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.

    08/15/2019

      Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.

      01/21/2018

        Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.

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