MMP-9 Fluorimetric Assay Kit


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MMP-9 Fluorimetric Assay Kit

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Cat.No. : Kit-3100
Product Overview : The MMP-9 Assay Kit provides a convenient assay for high throughput screening of MMP-9 inducers and inhibitors. It detects MMP-9 activity in a variety of biological samples using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET)5 peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon cleavage into two separate fragments by MMP-9 (Scheme 1), the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. Compared to an EDANS/DABCYL FRET substrate, this 5-FAM/QXL520 substrate provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
Storage : Store all kit Components at -20centigrade
Protect Components A and B from light and moisture
Components D and E can be stored at 4centigrade for convenience
Size : 100 Assays
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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