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Recombinant Rat MMP9 cell lysate

Cat.No. : MMP9-1940RCL
Product Overview : Rat MMP-9 / CLG4B derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : Mmp9 matrix metallopeptidase 9 [ Rattus norvegicus ]
Official Symbol : MMP9
Synonyms : MMP9; matrix metallopeptidase 9; matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase);
Gene ID : 81687
mRNA Refseq : NM_031055
Protein Refseq : NP_112317
Pathway : Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Endochondral Ossification, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; Matrix Metalloproteinases, organism-specific biosystem;
Function : fibronectin binding; metal ion binding; metalloendopeptidase activity; metalloendopeptidase activity; metalloendopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; metallopeptidase activity; peptidase activity; peptidase activity; protein binding; protein complex binding; zinc ion binding;

Not For Human Consumption!

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Customer Reviews (3)

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Reviews
05/03/2020

    The superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.

    08/15/2019

      Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.

      01/21/2018

        Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.

        Q&As (5)

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        What are the consequences of dysregulated MMP9 expression in cancer progression? 02/13/2022

        Dysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.

        What is the impact of MMP9 polymorphisms on enzyme activity? 09/07/2021

        Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.

        Can you explain the role of MMP9 in tissue remodeling at the molecular level? 01/17/2020

        MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.

        What is the role of MMP9 in the breakdown of the blood-brain barrier during neurological disorders? 11/14/2019

        MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.

        How does MMP9 contribute to angiogenesis at the molecular level? 06/02/2018

        MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.

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