CAB11623MH
Optional Service: Optional requirements on this protein
Product Overview :
Mouse Monoclonal Antibody to Human Ubiquitin Specific Peptidase 7
Antigen Description :
Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein which belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the reactivation of quiescent viral genomes. ICP0 interacts very strongly with USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP0-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP0 are expressed.
Specificity :
Human USP7 / HAUSP. No cross-reactivity with Baculovirus lysate in ELISA.
Immunogen :
Recombinant Human USP7 / HAUSP Protein
Host :
Mouse
Isotype :
Mouse IgG2b
Species :
Human
Clone :
8C10I3
Applications :
Western blot; ELISA
Dilution :
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human USP7 (208-560) in WB. Using a DAB detection system, the detection limit for Human USP7 (208-560) is approximately 100 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human USP7 (208-560). The detection limit for Human USP7 (208-560) is approximately 0.078 ng/well.
Preparation :
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human USP7 / HAUSP. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format :
0.2 μm filtered solution in PBS with 5% trehalose
Storage :
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.