Active Recombinant Human USP7 protein, His-tagged

• Cat.No.: USP7-922H
• Product Overview: Recombinant Human USP7 fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Description: Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family. It has a predicted molecular weight of 130 kDa. Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs. USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869. USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis. USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4. Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
• Bio-activity: Recombinant Human His6-USP7 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP7 concentration of 1-5 nM.
• Molecular Mass: 129 kDa
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
• Official Symbol: USP7
• Synonyms: USP7; ubiquitin specific peptidase 7 (herpes virus-associated); HAUSP, ubiquitin specific protease 7 (herpes virus associated); ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin specific protease 7 (herpes virus-associated); TEF1; HAUSP
• Gene ID: 7874
• mRNA Refseq: NM_003470
• Protein Refseq: NP_003461
• MIM: 602519
• UniProt ID: Q93009
• Chromosome Location: 16p13.3
• Pathway: FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; p53 pathway, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; p53 binding; peptidase activity; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity

Reviews

10/22/2021

The product's widespread recognition among researchers made it a preferred choice for many scientific studies.

06/17/2018

Positive feedback from other labs and researchers confirmed its standing as a valuable research tool.

06/28/2016

The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

Q&As

Does USP7 interact with specific protein substrates?

Yes, USP7 interacts with various protein substrates, including p53, MDM2, and PTEN, influencing their stability and cellular functions.

Can USP7 inhibitors be potential cancer therapeutics?

Inhibitors targeting USP7 are being explored as potential cancer therapeutics, aiming to disrupt the stability of oncogenic proteins and inhibit cancer cell growth.

Does USP7 have tissue-specific expression patterns?

USP7 exhibits tissue-specific expression patterns, and its levels may vary in different tissues and cell types.

What is the significance of USP7 in neurodegenerative diseases?

USP7 has been implicated in neurodegenerative diseases, and its dysregulation may contribute to protein aggregation and neuronal dysfunction.

Are there post-translational modifications of USP7?

Yes, USP7 undergoes post-translational modifications, including phosphorylation and ubiquitination, which can regulate its activity and stability.

How does USP7 regulate the p53 pathway?

USP7 regulates the p53 pathway by deubiquitinating and stabilizing p53, preventing its degradation and promoting its transcriptional activity.

Is USP7 involved in the immune response?

Yes, USP7 plays a role in the immune response by regulating the stability of proteins involved in immune signaling pathways.