Recombinant Human USP7 cell lysate

• Cat.No.: USP7-671HCL
• Product Overview: Human USP7 / HAUSP derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: USP7 ubiquitin specific peptidase 7 (herpes virus-associated) [ Homo sapiens ]
• Official Symbol: USP7
• Synonyms: USP7; ubiquitin specific peptidase 7 (herpes virus-associated); HAUSP, ubiquitin specific protease 7 (herpes virus associated); ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin specific protease 7 (herpes virus-associated); TEF1; HAUSP
• Gene ID: 7874
• mRNA Refseq: NM_003470
• Protein Refseq: NP_003461
• MIM: 602519
• UniProt ID: Q93009
• Chromosome Location: 16p13.3
• Pathway: FoxO family signaling, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; p53 pathway, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; p53 binding; peptidase activity; protein C-terminus binding; protein binding; protein homodimerization activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity

Reviews

10/22/2021

The product's widespread recognition among researchers made it a preferred choice for many scientific studies.

06/17/2018

Positive feedback from other labs and researchers confirmed its standing as a valuable research tool.

06/28/2016

The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

Q&As

Does USP7 interact with specific protein substrates?

Yes, USP7 interacts with various protein substrates, including p53, MDM2, and PTEN, influencing their stability and cellular functions.

Can USP7 inhibitors be potential cancer therapeutics?

Inhibitors targeting USP7 are being explored as potential cancer therapeutics, aiming to disrupt the stability of oncogenic proteins and inhibit cancer cell growth.

Does USP7 have tissue-specific expression patterns?

USP7 exhibits tissue-specific expression patterns, and its levels may vary in different tissues and cell types.

What is the significance of USP7 in neurodegenerative diseases?

USP7 has been implicated in neurodegenerative diseases, and its dysregulation may contribute to protein aggregation and neuronal dysfunction.

Are there post-translational modifications of USP7?

Yes, USP7 undergoes post-translational modifications, including phosphorylation and ubiquitination, which can regulate its activity and stability.

How does USP7 regulate the p53 pathway?

USP7 regulates the p53 pathway by deubiquitinating and stabilizing p53, preventing its degradation and promoting its transcriptional activity.

Is USP7 involved in the immune response?

Yes, USP7 plays a role in the immune response by regulating the stability of proteins involved in immune signaling pathways.