PDE1C TR-FRET Assay Kit
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Cat.No. : | Kit-1831 Optional Service: Optional requirements on this protein |
Product Overview : | Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader. |
Size : | 96 reactions |
Description : | The PDE1C TR-FRET Assay Kit is designed for identification of inhibitors of PDE1C using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. Using this kit, only two simple steps on a microtiter plate are required for the PDE1C activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then, fluorescence intensity can be measured using a fluorescence reader. |
Applications : | Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. |
Storage : | At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
◆ Recombinant Protein
PDE1C-3987R | Recombinant Rat PDE1C Protein, His (Fc)-Avi-tagged | +Inquiry |
PDE1C-047H | Recombinant Human PDE1C Protein, His/GST-tagged | +Inquiry |
PDE1C-03H | Recombinant Human PDE1C Protein, His/GST-tagged | +Inquiry |
PDE1C-4327R | Recombinant Rat PDE1C Protein | +Inquiry |
PDE1C-7362H | Recombinant Human PDE1C, His & GST tagged | +Inquiry |
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◆ Lysates
PDE1C-001HCL | Recombinant Human PDE1C cell lysate | +Inquiry |
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◆ Assay kits
Kit-1828 | PDE1C Assay Kit I | +Inquiry |
Kit-1830 | PDE1C Mouse Assay Kit | +Inquiry |
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